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The chlorophyll content determined by the Arnon filtration method is quite different from other methods. The main reason may be that the Arnon method requires filtration when determining the chlorophyll content, and the filtration process causes a partial loss of chlorophyll content. Arnon centrifugation is slightly more efficient than Arnon filtration. The efficiencies of the chlorophyll content determination by extraction method were as follows: 80% acetone extraction method <95% ethanol extraction method <three mixture solution extraction method <two mixture solution extraction method.
Since the chlorophyll has a process of outward diffusion from the tissue after the start of the leaching, the extracted chlorophyll increases continuously. When the extraction time was 5 h, the diffusion of chlorophyll into the extraction solvent reached equilibrium and the chlorophyll content in the solution reached the highest. After the leaching time exceeds 5 h, the chlorophyll content in the solution begins to decrease. The probable reason is that with the increase of the extraction time, the chlorophyll leakage of the plant takes longer time under light, and the instability of the light causes the chlorophyll content to decrease continuously.
If the use of chlorophyll detector can avoid the drawbacks of the appeal, the measurement results obtained are more accurate and more convincing in the study.
In the industrial market, the chlorophyll content in plants is extracted as a pigment, and in medicine, chlorophyll is a health food. The determination of the chlorophyll content in plants can be directly measured using a chlorophyll detector, and other methods can also be used. Measurements, such as the most common use of organic substances for the extraction of chlorophyll content, are measured. The following are the operating methods used for the determination after extraction with organic substances and the disadvantages of the determination process: