Vacuum freeze-drying preserved cornea has not been reported at home and abroad. Sucrose is difficult to dry when dry. According to the mechanism of the protective agent, we chose dimethyl sulfoxide (DMSO) as the protective agent inside the membrane and albumin as the protective agent outside the membrane. The purpose of drying is to remove the moisture in the corneal cells. After vacuum freeze-drying, the cornea can be easily aseptically packaged for long-term storage. The discharge of water during corneal drying is complicated. To ensure the activity of corneal cells in the drying process, controlling the temperature, pressure, and drying rate of the drying process are three important factors. After the cornea is freeze-dried, it needs rehydration before use. After the cornea is vacuum freeze-dried, the corneal tissue structure and cell morphology are preserved, but the water loss, various ions and many nutrients are lost, and the vacuum freeze-drying The rehydration of the cornea is definitely different from the rewarming of the cornea after cryogenic freezing. Experimental Study on Vacuum Freeze - drying of Cell Culture Medium and Preservation of Rabbit Corneal Endothelial Cells . The main function of corneal endothelial cells is to regulate the moisture in the cornea and maintain the transparency of the cornea. The corneal epithelium and endothelium were intact, smooth, transparent and no shedding after vacuum rehydration and corneal rehydration, and the whole cornea was free from edema and transparency. Microscopically, the corneal endothelial cells are hexagonal with clear boundaries. It is believed that the damage is mainly manifested by the cells being highly sensitive to mechanical or osmotic injury. Vacuum freeze-drying will cause greater damage to the endothelial cells of the cornea. Although the density and activity rate of the corneal endothelial cells after rehydration after vacuum freeze-drying is lower than that in the deep cryopreservation group, it is lower than normal rabbits, but it is still higher s level. Vacuum freeze-drying may cause greater damage to the endothelial cells of the cornea than cryogenic freezing. However, the results suggest that vacuum freeze-drying can maintain the activity of corneal endothelial cells. With the continuous improvement of the quality and performance of the vacuum freeze dryer, the gradual improvement of the structure, the corneal endothelial cell density and survival rate will increase, creating a long-term preservation of the cornea.
Henan Qianli Machinery CO. Ltd is a profession manufacture of Farm Tractor, rice machine, Reaper Machine, Power Tiller, garden machine and other farm implements. Our factory was established in 1987 and has more than 30 years of production and sales experience. We focus on providing customers with high-quality products and excellent services.
Reaper is mainly used to harvest crops such as wheat, corn, soybeans, etc. It can be matched with walking tractors, tillers, etc.
Reaper Head,Small Reaper Head,Power Tiller Reaper Head,Walking Tractor Reaper Head Henan Qianli Machinery Co.,Ltd. , https://www.hnchalion.com
Study on Vacuum Freeze-drying of Corneal Endothelial Cells
Core Tip: [Tags: SY_Introduction]